Topics
Theory
- Cell metabolism and growth a. Cell cycle i. phases ii. theoretical limit to growth rate b. Inputs (nutrients) c. Outputs (waste) d. Standard range
- Cell-cell interactions a. Autocrine, paracrine, endocrine, etc b. Lateral-inhibition and others c. Adherence and coherence
- Evolution a. Cancer b. Primary
- Boyancy
Practice
- Hydrodynamics a. Culture flask waste convection b. Shear stress during vortexing
- pH a. indicator solutions b. managing pH
- Free-radicals a. significance b. 2-mercapto-ethanol
- Nutrients a. NEAA b. Glycine c. Aspartic acid d. Serum
- Growth factors a. Serum proteins b. Cell types
- Attached vs suspended a. Enzymatic digestion b. Physical digestion
- Asepticity a. How not to mess up your cell line
Storage
- Freezing cells
- Record-keeping