Grill_Spinning_Disc_2

Info, drivers, documentation for Grill Spining Disc 2 in 454

Service notes

Changes to hardware and software are documented here.

June - July 2023

Triggerscope setup according to Austin Blanco/Nico Sturman published recommendations

YouTube videos here:

Setup in micro-manager: https://youtu.be/5AC9UtiF0JM
TTL and blanking: https://www.youtube.com/watch?v=7N5PMg5q5zI

TTL Control of Lasers and Camera

The Triggerscope has been set to act as a virtual shutter to control laser on-off and synchronize the lasers to the Orca camera. The current configuration also controls laser power (0 to 5 watts). This configuration allows different "channels" to use different laser powers, and so different laser powers can be set during a time series.

Blanking is on for the TTL control and set to high for both camera and active laser. That is, the laser AND the camera must be fully on for either to be active.

For faster operation, it is possible to configure only the laser on-off to be controlled by the virtual shutter. The power remains at whatever it is set to. This configuration does not allow power to be set within the channel, so laser power for a given laser cannot be changed during a time series. That is, if 488nm is set to 1V and 594nm is set to 0.5V, then all channels must use those laser powers for the entire time series.

For simplicity, the current default configuration only provides channels that include a laser power setting. In the channel.

TTL and DAC assignments

TTLs

TTL3: 488nm laser (GFP)
TTL5: 594nm laser (mKate/mCherry)
TTL8: Camera

DACs

DAC3: 488nm laser power (GFP)
DAC5: 594nm laser power (mKate)
DAC7: ASI Z-piezo

Early-June 2023

Original FCP8 fiber re-installed.

It turns out that the FCP8 was correctly specified. Original fiber back from Omicron (oops) and verified by Sebatsion (specifications), Archit and Jamie (imaging).
Omicron laser input optimized by Stephan to ~75mW at the fiber tip.
New dichroic for 488/594 GFP/mKate imaging: ZT488/594TPC 13x15x0.5mm Lot#413707
New emission filter for 488/594 GFP/mKate imaging: ZET488/594m 25mm die mounted Lot#412247
Filters from AHF Analysentechnik Dr. Michael Sommerauer

  Dr. Michael Sommerauer
  Sales Director Optical Filters
  AHF analysentechnik AG
  Kohlplattenweg 18
  DE -  72074 Tuebingen
  ms@ahf.de
  www.ahf.de
  fon: +49-7071/970901-15
  fax: +49-7071/970901-99

Old filters kept in the AHF box

Current filters in the Sutter Lambda 10B are now:

  4 Label,Wheel-A,9,9: block
  3 Label,Wheel-A,8,8: BF (empty)
  2 Label,Wheel-A,7,7: 488 LP
  1 Label,Wheel-A,6,6: polarizer
  0 Label,Wheel-A,5,5: BF
  9 Label,Wheel-A,4,4: block
  8 Label,Wheel-A,3,3: ZET488-594m
  7 Label,Wheel-A,2,2: 525/50
  6 Label,Wheel-A,1,1: BP
  5 Label,Wheel-A,0,0: 512/630 db

Micro-manager configuration file updated with GFP, mKate, and GFP/mKate channels.

Mid-May 2023

Hammamatsu camera revived with the help of Sebastian Bundschu and Archit. The status LED had been turned off in the software. The issue was getting the driver version matched to the software version of (µ-Manager? Hammamatsu control software?).
Andor laser combiner retired (Jamie White).
Omicron laser combiner with 445, 488, 515, and 594nm lasers commissioned (Jamie White).
Original FCP8 fiber that arrived with Omicron exchanged by Omicron for an AFP fiber. See Fiber_exchange in Omicron folder. This chage was instigated after Pete Pitrone from Andor. (Turns out he was incorrect; see below).
Triggerscope ordered for laser triggering and power control.
Filters ordered for 488/594 dual-excitation imaging. A new dichroic in the CSU-X1 head blocks detection of both laser lines, and a dual-band emission filter allows the GFP and mKate2 signals to pass to the camera. Dual-color imaging is achieved by switching the lasers without switching the emission filters.
Archit improvised laser control with an Arduino.
Archit switched to using the Andor Precision Control Unit for laser control: lasers are triggered by the Orca camera

Early May 2023

Pete Pitrone service visit:

optimized laser output in the Andor combiner.
488 to ~5mW and 561 to ~15mW
561 laser well beyond rated service life.
488 relatively new but still weak.
CSU-X1 aligned (didn't need it) and cleaned (didn't nead it).
Hammamatsu camera failed.

April 2023

Relocated from 256 to 454 Optics suite (Jamie White).
Cable connections and port assignments documented.
Laser power low. 488 unuseable (~1mW), 561 barely (~5mW).

jqwhite/Grill_Spinning_Disc_2