BIOS9000

This repo contains the course materials for a 2 day class on assembly. This course is run on infrastructure provided by the University of Oslo.

Shedule

Day 1

  • Group activity: intro assembly exercise
  • Discussion assembly exercise
  • Lecture: intro to assembly, principles and problems
  • Group activity: de Bruijn graphs by hand
  • Break
  • Lecture: quick intro to assembly with velvet
  • Class activity: a first velvet assembly
  • Class activity: assembly statistics discussion
  • Group activity: Starting Unicycler

Day 2

  • Group activity: starting SPAdes and Flye assemblers
  • Lecture: on SPAdes, Flye and Unicycler
  • Class activity: evaluation without a reference
  • Break
  • Lecture: Comparing to a reference
  • Class activity: comparing assemblies - velvet and SPAdes
  • Group activity: comparing assemblies with quast