barcode has wrong length
ZHIDIHUAYUAN opened this issue · 2 comments
ZHIDIHUAYUAN commented
Hi,
Libraries were prepared with 10X single cell 3' v2 kit. When I ran dropest , the output was :
Start merge: 00:57:28. Merge initialized: 00:57:28. Barcode 'ACGAGGATCTAAGCCA' has wrong length (24 expected).
My config was:
I don't know what was wrong with my code. please help me. thank you.
ZHIDIHUAYUAN commented
Sorry, I had another questions.
After I align the reads using hisat2, should I filter the bam file according to the 'NH:i:1' ?
ZHIDIHUAYUAN commented
Hi，
Maybe I should use 10x_aug_2016_split. But, should I only use uniquely mapped reads to count?