Dropest for 10x v3 chemistry
dalhoomist opened this issue · 2 comments
dalhoomist commented
Hi,
10x released v3 chemistry recently. Do I need to do something different with the dropest pipeline compared with the V2 chemistry? Is the 10x_aug_2016_split file good for the barcodes?
From what I can gather, the 10x V3 has 12bp UMI but still a 16bp cell barcode.
Thanks
Osama
rrydbirk commented
Indeed, v3 has 12bp UMI, so this needs to be changed in the config:
<umi_length>12</umi_length>
dalhoomist commented
That is what I thought. Thank you for the help.