/Aging_human_dermal_fibroblast_nucleus

human dermal fibroblast nucleus DAPI of various age cell lines

Apache License 2.0Apache-2.0

Aging_human_dermal_fibroblast_nucleus_DAPI

This image repository is for Nature Protocol paper(2020): A robust unsupervised machine learning method to quantify the morphological heterogeneity of cells and nuclei

Dataset Size: 1.96GB (Download time ~10min @ 100Mbps)

Sample Size:
7 Ages : 3, 29, 35, 45, 55, 85, 96
2 Technical repeat
36 Images per repeat
7 x 2 x 36 = 504 images total

Cell Count: 2300 cells
AG04054_1 = 144
AG04054_2 = 143
AG04059_1 = 213
AG04059_1 = 251
AG08904_1 = 163
AG08904_1 = 187
AG09162_1 = 100
AG09162_2 = 131
AG09558_1 = 254
AG09558_2 = 243
AG11796_1 = 137
AG11796_2 = 127
GM05565_1 = 107
GM05565_2 = 100

Analysis time with i9-10900K @4.8Ghz, CellProfiler 3.1.9, Windows 10
CellProfiler segmentation: 10min
Boundary detection from segmented image: 5min
Building the model: 4sec
Applying the model: 10sec