Combining Pacbio and nanopore

Question

Combining Pacbio and nanopore

Closed this issue 5 years ago · 4 comments

Deleted user commented 5 years ago

Hello,
how do we feed the 2 types of reads to Ra, for a single assembly?

thank you

Answer 1 · 2019-08-14T13:02:30.000Z

Hello,
two types as in ONT and PB, or either of those plus Illumina?

Best regards,
Robert

Answer 2 · 2019-08-14T13:04:17.000Z

Hello,
actually for my organism I have
Pacbio data
ONT data
illumina data

So I was planning PacBio + ONT for assembly, and illumina for Racon polishing.

Answer 3 · 2019-08-14T13:05:44.000Z

You can merge the PB and ONT FASTA/Q files, run with -x ont if there are more ONT reads (-x pb otherwise). The command will then be: ra -x ont <combined tgs> <illumina> > assembly.fasta.

Answer 4 · 2019-08-14T13:10:20.000Z

Perfect, thank you!