Combining Pacbio and nanopore
Closed this issue · 4 comments
Deleted user commented
Hello,
how do we feed the 2 types of reads to Ra, for a single assembly?
thank you
rvaser commented
Hello,
two types as in ONT and PB, or either of those plus Illumina?
Best regards,
Robert
Deleted user commented
Hello,
actually for my organism I have
Pacbio data
ONT data
illumina data
So I was planning PacBio + ONT for assembly, and illumina for Racon polishing.
rvaser commented
You can merge the PB and ONT FASTA/Q files, run with -x ont if there are more ONT reads (-x pb otherwise). The command will then be: ra -x ont <combined tgs> <illumina> > assembly.fasta
.
Deleted user commented
Perfect, thank you!