Precompiled binaries are available on Figshare:
Alternatively, you can build tablemaker from source by following these instructions. (The process is the same as building Cufflinks from source). Source code is in the tablemaker-2.1.1 directory.
Note: Tablemaker relies on Cufflinks version 2.1.1 (released April 2013) to estimate transcript FPKMs, and these estimates may not match FPKM estimates obtained from Cufflinks 2.2.0 (released March 2014) or 2.2.1 (released May 2014). We are actively working on integrating better with Cufflinks.
Please report any problems as issues on this repository.
Users can run Tablemaker to organize assembly output from the likes of Cufflinks into a format that Ballgown can load.
Tablemaker needs to be run on each RNA-seq sample in your experiment. It requires one single transcriptome assembly in GTF format for a single experiment containing multiple samples. Tablemaker also requires read alignments for each sample in BAM format. From the command line, Tablemaker is run as follows:
tablemaker -p 4 -q -W -G merged.gtf -o sample01_output sample_01/accepted_hits.bam
where:
-pdenotes how many threads to use (the program can take a few hours to run, but can be parallelized)- The
-qcan be removed for more verbose output messages -Wand-G merged.gtfare required. The-Wtells the program to run in tablemaker mode (rather than Cufflinks mode), and the-Gargument points to the assembly GTF file, which gives the assembled transcripts' structures. For Cufflinks users, often this is themerged.gtfoutput from Cuffmerge.- The argument to
-ois the desired output directory for the sample (each sample should have its own output directory) - The read alignment file is the last argument. If reads were aligned with TopHat, this is usually some variant of
accepted_hits.bam