lh3/miniasm

High scoring segment pairs

rehab-fouad opened this issue · 5 comments

How to calculate high scoring segment pairs with minimap2 for nanopore cDNA reads?

lh3 commented

What do you mean by "high scoring segment pairs"? What do you want to do?

I want to know the length and numbers of segment in per read that mapped to the reference without gaps or mismatches

lh3 commented

Sorry. You can't do that with minimap2.

at the link you sent me about fap
https://lh3.github.io/minimap2/minimap2.html#10
there is a description of ms; which is the maximum scoring segment. but in the output file, I got I don't get this column. do you have any idea how can I get this?

or which tool I can use to get this value from minimap output file?