lilit-nersisyan/fivepseq

Issues with GTF annotation files

sgrannem opened this issue · 1 comments

I am running the pipeline sing python 3.9
I was able to successfully run the pipeline using a gff file but I had to manually add the biotypes to column 8 in the file.
However, when I used a gtf file, I could not get the pipeline to work even when I manually added the biotypes to the file. It doesn't seem to process the gtf file correctly in python 3

It would be helpful if you could share a few lines from the GTF file you modified.

I ran fivepseq with Ensembl GTF with no issues.