This repository contains configuration files for the Illumina® Experiment Manager (IEM) for third-party indices. Adding these files to your IEM configuration will allow you to create SampleSheets using IEM for third-party library kits.
The configuration files were created for use with IEM 1.19.1 following the instructions in the IEM Software Guide from January 2021.
The files are provided as a courtesy to users of the varvis® software. They are free to use for anybody else. Feedback or feature requests are always welcome.
The configuration files are provided "as is" and the authors make no representations or warranties of any kind concerning suitability, correctness or functionality. These files are not part of the medical device varvis®.
Links to other websites are provided as a convenience and do not indicate any endorsement or business relationship.
Please download the latest version from releases. ZIP archives containing all configuration files are provided there.
IEM is typically installed into a directory such as this:
C:\Program Files (x86)\Illumina\Illumina Experiment Manager
The IEM program directory contains four directories:
Applications
Genomes
IndexKits
SamplePrepKits
Copy the files from the directories in this repository into the respective directory of IEM on your computer. Then, restart IEM.
Note that you will probably need administrator rights on your computer in order to modify files in the program directory.
| Name | Manufacturer | Length/ nt | SamplePrepKit | IndexKits |
|---|---|---|---|---|
| IDX96 | Nimagen | 10 | Nimagen UDI |
Nimagen IDX96-U01Nimagen IDX96-U02v2Nimagen IDX96-U03Nimagen IDX96-U05Nimagen IDX96-U06 |
| IDX96 | Nimagen | 8* | Nimagen UDI |
Nimagen IDX96-U01-8ntNimagen IDX96-U02v2-8ntNimagen IDX96-U03-8ntNimagen IDX96-U05-8ntNimagen IDX96-U06-8nt |
| IDX96 | Nimagen | 8* | Nimagen UDI |
Nimagen IDX96-U01-RC-8ntNimagen IDX96-U02v2-RC-8ntNimagen IDX96-U03-RC-8ntNimagen IDX96-U05-RC-8ntNimagen IDX96-U06-RC-8nt |
| 16-UDI | Twist Bioscience | 10 | Twist Enrichment UDI |
Twist 16 UDI |
| 96-UDI | Twist Bioscience | 10 | Twist Enrichment UDI |
Twist 384 UDITwist 96 UDI Plate ATwist 96 UDI Plate BTwist 96 UDI Plate CTwist 96 UDI Plate D |
| IDT 8nt | IDT | 8 | IDT UDI 8nt UMI |
IDT UDI 8nt UMI Set AIDT UDI 8nt UMI Set BIDT UDI 8nt UMI Set CIDT UDI 8nt UMI Set DIDT UDI 8nt UMI 384 Indices |
*Some Nimagen amplicon designs require to use at least 151 cycles for Read 1 and Read 2. This is not compatible with 10nt indices on most devices. Nimagen states, however, that their 10nt index plates can be used as 8nt indices. For convenience, we created configurations with 8nt from those index plates. Please note that you must select different configurations depending on your device, for example:
Nimagen IDX96-U01-8ntfor the Forward Strand Workflow implemented in MiSeq and NovaSeq (v1.0 reagents),Nimagen IDX96-U01-RC-8ntfor the Reverse Complement Workflow implemented in NextSeq and NovaSeq (v1.5 reagents).
See also the Indexed Sequencing Overview Guide Document #15057455 (v08 Nov 2020).
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