MinKNOW is Oxford Nanopore Technologies Device Control software embedded in MinIT, GridION, PromethION and provided for installation on user PCs to run MinIONs. It carries out several core tasks: data acquisition, real-time analysis and feedback, basecalling, data streaming, device control (including selecting the run parameters), sample identification and tracking, and ensuring that the platform chemistry is performing correctly to run the samples.
The files and code in this repository provide a way of automating interactions with MinKNOW, including gathering information about running or finished experiments, starting and stopping those experiments, and even exerting more fine-grained control. Anything that can be done via MinKNOW's user interface (and more) can be done using the APIs in this project.
This project is targetted at LIMS developers or developers of other tools that have a need to integrate with MinKNOW. Some familiarity with Python is expected (although the APIs can be used from other languages), as the examples are written in Python. If you are not familiar with gRPC, it is worth reading through some of the gRPC documentation to get a feel for it.
The first thing you will need is an installation of MinKNOW to communicate with. This can be obtained from the Oxford Nanopore Community download pages if you want a local installation, although the installation on a GridION, PromethION, Mk1C, etc will also work.
Note that you will need a compatible version of MinKNOW for the version of minknow_api you are
using - see the FAQs below.
For the Python module and examples, you will also need Python 3.5 or later, with the following Python packages installed:
The minknow_api Python package provides a client library for the MinKNOW APIs. The recommended way
to get it is from PyPI using pip, but it can also be built from source (see BUILD.md).
# Install minknow_api
> pip install minknow_api
# Verify API is installed correctly (from a checkout of this repository):
> python ./python/minknow_api/examples/list_sequencing_positions.py --host localhost --port 9501
# Possible output if running minknow locally:
# Available sequencing positions on localhost:9501:
# MN12345: running
# secure: 8000The package contains plenty of documentation in its docstrings, although for an overview of the
MinKNOW APIs themselves you may prefer to read the API description files -
see below for further discussion of these files. There are also examples (like
the list_sequencing_positions.py script in the above instructions) that show how to perform some
common tasks.
MinKNOW's API is based on gRPC and can be used from any language supported by gRPC. This
includes Go, Java, Ruby, JavaScript (although see the note below about JavaScript) and many more.
The gRPC documentation describes how to generate client libraries from the API
descriptions in this repository, and how to use those libraries. The .proto
files themselves contain documentation about what the various RPCs do, and a more general overview
is given below.
The Manager interface is available on port 9502 - see the FAQs for more details.
From there, APIs are available to get the ports that other services operate on (including the services
for each flow cell position).
How to use gRPC from JavaScript depends on whether you're using it from Node (in which case it works much like any other language - see above) or from a web browser environment. For web browsers (including Electron), you need to use the Web variant, which may also involve connecting to a different port.
Secure connections still start at 9502 for the Manager service. When using the Manager APIs to
get ports for other services, you should use fields with grpc_web in the name, like secure_grpc_web.
The files in protos/minknow_api describe MinKNOW's APIs. Each file
describes a single service, which covers a specific area of MinKNOW's functionality.
There are two global services, the manager service and basecaller service. There is only one instance of each of these services: see below for how to connect to them. All other services are provided by each flow cell position independently. For example, if you are using a GridION X5, which has 5 flow cell positions, there will be 5 ports (or sets of ports - secure, gRPC Web, etc), each of which will provide all the other services.
manager.proto is the entry point of MinKNOW's APIs. It is always
available on a specific port 9502.
The most important method is flow_cell_positions, which provides information about how to connect
to the services for each flow cell position. From there you can access all the flow cell
position-specific services.
Other methods on the manager service provide general information about the MinKNOW installation and its high-level state, as well as port information for the basecaller service service, which can be used to basecall data from previous experiments.
instance.proto provides general information about the flow cell
position. Of particular interest is the get_output_directories method, which indicates where data
files will be written to. set_output_directories can be used to change this.
stream_instance_activity may also be useful, as it provides a continuously-updated summary of the
state of the position.
This can be accessed via the ports reported by flow_cell_positions on the manager service.
protocol.proto allows starting and stopping experiment protocols, as well as providing information about the current and previous protocol runs. Note that information about protocol runs from before the last restart is not available via this API.
See the start_protocol example for an example of how to use
this service to start a protocol.
The main work of a protocol is acquiring data, and this is managed in
acquisition.proto. While most of the methods in
acquisition.proto will not be useful to most external tools, get_acquisition_info is helpful for
access detailed information about what was done by a protocol run reported by protocol.proto.
device.proto provides more detailed information about the
hardware of the flow cell position and the inserted flow cell. get_device_info provides some
constant information about the position, while get_flow_cell_info provides information about the
flow cell (a streaming version that provides updates about changes to the flow cell, such as it
being removed, is also available). It is also possible to override the flow cell identifier and
product code via this service (although this is not generally recommended).
statistics.proto provides statistics about current and previous protocol runs, including duty time and temperature information. This is useful for generating reports or tables of data describing how well an experiment has performed.
© 2021 Oxford Nanopore Technologies PLC.
API Specifications for MinKNOW is distributed under the Terms and Conditions of the Nanopore Community.
MinKNOW's API changes over time, to support new features and occasionally for other reasons, such as improving security. The important version is that of MinKNOW Core - this version looks like 3.6.5 or 4.0.1 - rather than the date-based version of the entire MinKNOW release (which looks more like 19.12.5).
Every minor release of MinKNOW Core (the 4.0 in 4.0.1) has a fixed API, which is described by the
corresponding minor releases of minknow_api. So you should use the latest 4.0.x release of
minknow_api to talk to MinKNOW Core 4.0.1.
We aim to keep API backward-compatibility within a major release (the 4 in 4.0.1) of MinKNOW Core.
This means that if you write code that talks to MinKNOW Core 4.2, say, using minknow_api 4.2.1, it
should work with MinKNOW 4.3, 4.4, etc (without even updating minknow_api), but it won't work
with MinKNOW Core 3.6 or 5.0. There is also no guarantee it will work with MinKNOW Core 4.0 or 4.1
(depending on which specific APIs you have used).
There is one standard port that MinKNOW exposes, which provides the manager service:
9502can be used with a gRPC or gRPC-Web "secure channel"
gRPC-Web is only used for browser-based clients; all other client should use the normal gRPC ports.
Ports reported by manager RPCs follow a similar pattern: there are two fields. For example, the
basecaller_api RPC returns a response with two fields:
secureis a port that can be connected to with a gRPC secure channelsecure_grpc_webis a port that can be connected to with a gRPC-Web secure channel
It may be that the secure and secure_grpc_web fields contain the same port number, but this is
not guaranteed and should not be relied on.
MinKNOW installations use a self-signed certificate for their secure ports. This means that the client library you use has to trust this certificate.
If you are using the minknow_api Python package, this is all handled for you. If you are using
minknow_api.manager.Manager, secure connections are used by default. This can be overridden with
the use_tls argument when creating this class. minknow_api.Connection can also be passed a
use_tls argument.
If you are using the gRPC client libraries directly (for example, if you are connecting from a language other than Python), you will need to tell the library about MinKNOW's TLS certificates.
Within the MinKNOW installation, you can find the CA certificate at conf/rpc-certs/ca.crt. This
can be passed to most gRPC client libraries as part of creating a secure/SSL channel.
Note that this certificate is only valid for the "localhost" name - connecting to 127.0.0.1
directly will not work, nor will connecting across the network. You can work around this by
setting the grpc.ssl_target_name_override channel option to localhost.
An acquisition period or acquisition run is a period in which data was being actively read from the flow cell. A typical protocol will have two acquisition periods - a short one with minimal analysis to calibrate the flow cell followed by a much longer one to actually gather sequence data.
When the APIs refer to a device, this is the same as a flow cell position. Newer APIs use the more descriptive flow cell position, but there are plenty of older APIs that use device instead.
In the user interface, an experiment name is another name for a protocol group ID (see Protocol). Note that what a user considers an "experiment" may not map onto a consistent concept in MinKNOW, and the APIs generally avoid this term in favour of a more precise term like "protocol run" or "protocol group".
The flow cell is a consumable that plugs into the sequencing unit and contains the chemistry required to sequence the sample. Often, but not always, contains some core electronics.
The flow cell position is the location that a flow cell can be plugged in to. It is synonymous with device in the APIs.
A protocol is a description of how to perform an experiment. This takes the form of some configuration and a Python script. A protocol run is a specific execution of that protocol.
A protocol group is a set of protocol runs given the same name (referred to as a "protocol group ID" in the API, and an "experiment name" in the user interface).
If you see the following error when connecting to the local machine:
grpc._channel._InactiveRpcError: <_InactiveRpcError of RPC that terminated with:
status = StatusCode.UNAUTHENTICATED
details = "Bad metadata key"
you can try setting the MINKNOW_API_USE_LOCAL_TOKEN environment variable to 1. Note that you
will need to have MinKNOW's local guest mode enabled for this to work (it is enabled by default, but
the setting can be changed via Mooneye).
If you see the following error when connecting to a remote machine:
grpc._channel._InactiveRpcError: <_InactiveRpcError of RPC that terminated with:
status = StatusCode.UNAUTHENTICATED
details = "Invalid local auth token"
you can try setting the MINKNOW_API_USE_LOCAL_TOKEN environment variable to 0.
If you get a MissingMinknowSSlCertError exception, try setting the MINKNOW_TRUSTED_CA
environment variable. This should be the path to the conf/rpc-certs/ca.crt file found in a MinKNOW
installation. You should use a copy of the file from the same version of MinKNOW as the one you are
attempting to connect to.