The NCBI SRA (Sequence Read Archive)

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Change Log

Please check the CHANGES.md file for change history.

The SRA Toolkit

The SRA Toolkit and SDK from NCBI is a collection of tools and libraries for using data in the INSDC Sequence Read Archives.

ANNOUNCEMENT:

February 10, 2022 : SRA Toolkit 3.0.0

NCBI's SRA changed the source build system to use CMake in toolkit release 3.0.0. This change is an important step to improve developers' productivity as it provides unified cross platform access to support multiple build systems. This change affects developers building NCBI SRA tools from source. Old makefiles and build systems are no longer supported.

This change also includes the structure of GitHub repositories, which underwent consolidation to provide an easier environment for building tools and libraries (NGS libs and dependencies are consolidated). Consolidation of NGS libraries and dependencies provides better usage scope isolation and makes building more straightforward.

Affected repositories

ncbi/ngs

This repository is frozen. All future development will take place in GitHub repository ncbi/sra-tools (this repository), under subdirectory ngs/.

ncbi/ncbi-vdb

This project's build system is based on CMake. The libraries providing access to SRA data in VDB format via the NGS API have moved to GitHub repository ncbi/sra-tools.

Old (base URL: https://github.com/ncbi/ncbi-vdb)	New (base URL: https://github.com/ncbi/sra-tools)
`libs/ngs`	`ngs/ncbi/ngs`
`libs/ngs-c++`	`ngs/ncbi/ngs-c++`
`libs/ngs-jni`	`ngs/ncbi/ngs-jni`
`libs/ngs-py`	`ngs/ncbi/ngs-py`
`libs/vdb-sqlite`	`libs/vdb-sqlite`
`test/ngs-java`	`test/ngs-java`
`test/ngs-python`	`test/ngs-python`

ncbi/sra-tools (This repository)

This project's build system is based on CMake. The project acquired some new components, as listed in the table above.

October 25, 2021. SRA Toolkit 2.11.3:

fixed a bug in fasterq-dump: fasta and fasta-unsorted parameters work correctly.

October 7, 2021. SRA Toolkit 2.11.2:

SRA data are now available either with full base quality scores (SRA Normalized Format), or with simplified quality scores (SRA Lite), depending on user preference. Both formats can be streamed on demand to the same filetypes (fastq, sam, etc.), so they are both compatible with existing workflows and applications that expect quality scores. However, the SRA Lite format is much smaller, enabling a reduction in storage footprint and data transfer times, allowing dumps to complete more rapidly. The SRA toolkit defaults to using the SRA Normalized Format that includes full, per-base quality scores, but users that do not require full base quality scores for their analysis can request the SRA Lite version to save time on their data transfers. To request the SRA Lite data when using the SRA toolkit, set the "Prefer SRA Lite files with simplified base quality scores" option on the main page of the toolkit configuration- this will instruct the tools to preferentially use the SRA Lite format when available (please be sure to use toolkit version 2.11.2 or later to access this feature). The quality scores generated from SRA Lite files will be the same for each base within a given read (quality = 30 or 3, depending on whether the Read Filter flag is set to 'pass' or 'reject'). Data in the SRA Normalized Format with full base quality scores will continue to have a .sra file extension, while the SRA Lite files have a .sralite file extension. For more information please see our data format page.

August 17, 2021: SRA Toolkit 2.11.1.

March 15, 2021: SRA Toolkit 2.11.0.

December 16, 2020: SRA Toolkit 2.10.9.

June 29, 2020: SRA Toolkit 2.10.8.

May 20, 2020: SRA Toolkit 2.10.7.

May 18, 2020: SRA Toolkit 2.10.6.

April 1, 2020: SRA Toolkit 2.10.5.

February 26, 2020: SRA Toolkit 2.10.4.

February 18, 2020: SRA Toolkit 2.10.3.

Release 2.10.2 of sra-tools provides access to all the public and controlled-access dbGaP of SRA in the AWS and GCP environments (Linux only for this release). This vast archive's original submission format and SRA-formatted data can both be accessed and computed on these clouds, eliminating the need to download from NCBI FTP as well as improving performance.

The prefetch tool also retrieves original submission files in addition to ETL data for public and controlled-access dbGaP data.

With release 2.10.0 of sra-tools we have added cloud-native operation for AWS and GCP environments (Linux only for this release), for use with the public SRA. prefetch is capable of retrieving original submission files in addition to ETL data.

With release 2.9.1 of sra-tools we have finally made available the tool fasterq-dump, a replacement for the much older fastq-dump tool. As its name implies, it runs faster, and is better suited for large-scale conversion of SRA objects into FASTQ files that are common on sites with enough disk space for temporary files. fasterq-dump is multi-threaded and performs bulk joins in a way that improves performance as compared to fastq-dump, which performs joins on a per-record basis (and is single-threaded).

fastq-dump is still supported as it handles more corner cases than fasterq-dump, but it is likely to be deprecated in the future.

You can get more information about fasterq-dump in our Wiki at https://github.com/ncbi/sra-tools/wiki/HowTo:-fasterq-dump.

For additional information on using, configuring, and building the toolkit, please visit our wiki or our web site at NCBI

SRA Toolkit Development Team