Long read support please?

[ZarulHanifah]

I am doing shotgun metagenome on ONT, assembling using metaFlye. Usually to access all sorts of microbes in the sample, the extraction method is rigorous that it would shear the gDNA, even with long reads, you would produce fragmented MAGs, maybe less 100 contigs per MAG. Jorg would be great if it is possible to complete the MAG with existing data.

[lmlui]

Please take a look at this preprint: https://doi.org/10.1101/2023.06.30.547288 It has a similar method as Jorg for long read metagenomes. We currently aren’t planning on turning this into a tool, but you can see the programs needed and try it on your own.

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On Wed, Apr 3, 2024 at 00:40 ZarulHanifah ***@***.***> wrote: I am doing shotgun metagenome on ONT, assembling using metaFlye. Usually to access all sorts of microbes in the sample, the extraction method is rigorous that it would shear the gDNA, even with long reads, you would produce fragmented MAGs, maybe less 100 contigs per MAG. Jorg would be great if it is possible to complete the MAG with existing data. — Reply to this email directly, view it on GitHub <#13>, or unsubscribe <https://github.com/notifications/unsubscribe-auth/AFP2762X2GGIOXB5NYIYAS3Y3OW77AVCNFSM6AAAAABFUYGL72VHI2DSMVQWIX3LMV43ASLTON2WKOZSGIZDEMJUHE2TKOA> . You are receiving this because you are subscribed to this thread.Message ID: ***@***.***>