Create figure illustrating the fundamental problem
mikemc opened this issue · 1 comments
mikemc commented
Normalization imposes competition, which causes the abundance of a focal taxon to depend on the relative abundances of all taxa. Perhaps have a panel that illustrates the experimental workflow, showing different types of normalization that can occur (saturating extraction yield; deliberate library normalization; computational normalization to proportions). Then have another panel (or two) showing that the error in the proportion (or abundance?) of a particular taxon depends on the composition of the rest of the sample, similar to Figure 2 of https://elifesciences.org/articles/46923