/scRiboSeq_manuscript

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scRibo-seq

This repository contains the collection of scripts to analyze raw single-cell ribosomal profiling data and generate the figures presented in the manuscript.

VanInsberghe, M., van den Berg, J., Andersson-Rolf, A., Clevers, H., van Oudenaarden, A., Single-cell Ribo-seq reveals cell cycle-dependent translational pausing. Nature (2021). https://doi.org/10.1038/s41586-021-03887-4

The repository is organized in three subfolders:

1. data_processing

Contains the NextFlow pipeline to process raw fastqs, creating QC plots, count tables, pre-processed alignments, and apply the random-forest correction.

2. random_forest

Contains the R scripts to prepare, train, and tune a random-forest model to correct for the MNase sequence bias. Additionally contains the tuned model used to correct the MNase sequence bias.

3. figures

Contains the R scripts to main the subfigures for the manuscript.

4. processing_config

Contains the nextflow configuration files and any modifications to the standard workflow for each data set.