nanoporetech/dorado

command works on 0.7.0 but error in 0.8.0

abcdtree opened this issue · 1 comments

Hi,

Issue Report

The same command below works well in 0.7.0, but will return error in 0.8.0

dorado basecaller ${model_home}/dna_r10.4.1_e8.2_400bps_sup@v5.0.0 ${data_home}/pod5 --estimate-poly-a --kit-name SQK-PCB114-24 > my.bam

##error message
terminate called after throwing an instance of 'std::invalid_argument'
  what():  Trim interval 118-93 is invalid for sequence TGTTATTGTACTGTACTTGGTTCAGTTATTTTGTGTATGCAAACGATATTAGAGGAAGAGTGAGCACCGTGACAACTAGGCACAGCGAGTCTCGTTTTCTGCTTGGTGCTGGATCTGCTGAACTC
/var/spool/slurm/job66442714/slurm_script: line 30: 114723 Aborte

Run environment:

  • Dorado version: 0.8.0
  • Dorado command: dorado basecaller ${model_home}/dna_r10.4.1_e8.2_400bps_sup@v5.0.0 ${data_home}/pod5 --estimate-poly-a --kit-name SQK-PCB114-24 > my.bam

my purpose is to test the difference on polyA tail estimation from version 0.7.0 and 0.8.0, anything I need to change for 0.8.0?

Thanks

Josh

This appears to be the same as #1020, please follow that issue for updates.