Modification tags and re-calling

[wanghlv]

Hi, I'm wondering if I'd like to call all modification possible using the rna004_130bps_sup@v5.1.0, will DORADO write out each modification separately in a different field in BAN file or output as different files. Thanks! Also, if I would like to redo-modification call later, do I need to start from POD5 files again? Thanks again!

[malton-ont]

Hi @wanghlv,

Modification data is stored in the output BAM file as MM and ML tags - see the docs here and the specification here (section 1.7) for more details.

If you want to recall the modifications you will need to re-basecall from pod5, yes.