zmq.error.Again: Resource temporarily unavailable

[Yoshi-Ochiai]

Hi,
Thank you for developing the m6Abasecaller model!

I try to run by option 1 with Guppy v3.4.5 and Singularity v3.7.0 but encountered the following error.
How can I solve the error? Thank you.

command

singularity exec --nv modPhred/modphred-3.6.1_latest.sif modPhred/run \
-c path/to/config/rna_r9.4.1_70bps_m6A_hac.cfg \
--host guppy_basecall_server \
-f path/to/rna_sequence.fa \
-o m6abasecaller \
-i /path/to/fast5_pass/

output and error messages

[2023-12-05 16:31:25] ===== Welcome, welcome to modPhred pipeline! =====
[2023-12-05 16:31:25] Connecting to: guppy_basecall_server:5555 ... [mem: 134 MB]
[2023-12-05 16:31:25] Encoding modification info from 1 directories... [mem: 134 MB]
[2023-12-05 16:31:25] /path/to/fast5_pass/ with 28 Fast5 file(s)... [mem: 134 MB]
multiprocessing.pool.RemoteTraceback:
"""
Traceback (most recent call last):
File "/opt/miniconda3/lib/python3.9/multiprocessing/pool.py", line 125, in worker
result = (True, func(*args, **kwds))
File "path_to/modPhred/src/guppy_encode_live.py", line 109, in basecalling_worker
client.connect()
File "/opt/miniconda3/lib/python3.9/site-packages/pyguppyclient/client.py", line 73, in connect
config = self._load_config(self.config_name)
File "/opt/miniconda3/lib/python3.9/site-packages/pyguppyclient/client.py", line 95, in _load_config
loaded_configs = {Config(c).name: Config(c) for c in self.get_configs()}
File "/opt/miniconda3/lib/python3.9/site-packages/pyguppyclient/client.py", line 91, in get_configs
res = self.send(SimpleRequestType.GET_CONFIGS)
File "/opt/miniconda3/lib/python3.9/site-packages/pyguppyclient/client.py", line 55, in send
return simple_response(self.recv())
File "/opt/miniconda3/lib/python3.9/site-packages/pyguppyclient/client.py", line 65, in recv
raise Again()
zmq.error.Again: Resource temporarily unavailable

[lpryszcz]

Hi, could you please try with guppy v3.6.1?

[Yoshi-Ochiai]

Hi lpryszcz,
I did run Guppy v3.6.1 again, but the same error message appeared.

[enovoa]

hi @Yoshi-Ochiai we are planning to embed modPhred into MasterOfpores NextFlow workflow, to ensure that there are no errors caused by the use of different software versions/dependencies. We hope to have this set up in a couple of weeks and will update the README accordingly. Thanks!

[Yoshi-Ochiai]

Hi @enovoa
OK. I look forward to the update! Thank you!