Nothing output in bam file when running step 3. RNA modification stoichiometry estimation using Tombo resquiggling
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Renown-TAL commented
Hello!
I tried this step(step 3. RNA modification stoichiometry estimation using Tombo resquiggling) using data(RNA574356_WT45C/workspace/batch0.fast5 and RNA574356_WT30C/workspace/batch0.fast5) with code ( ~/software/nanoRMS/per_read/get_features.py --rna -f Saccharomyces_cerevisiae.R64-1-1_firstcolumn.ncrna.fa -t 25 -i batch0.fast5), the output bam file have nothing but the sam type header without any warning.
Could you tell me what happend?
Thank you in advance.