ntm/cox12_evolutionExperiment

Scripts used to analyze the data from Fabien Pierrel's delta-cox12 project

This repository contains code developed for the bioinformatics analyses underlying our manuscript provisionally entitled:
The PSI+ prion and HSP104 modulate the cytochrome c oxidase deficiency caused by deletion of COX12
by Pawan Kumar Saini, Hannah Dawitz, Andreas Aufschnaiter, Jinsu Thomas, Amélie Amblard, James Stewart, Nicolas Thierry-Mieg, Martin Ott and Fabien Pierrel.
https://www.biorxiv.org/content/10.1101/2021.10.08.463630v2

The scripts below are listed in execution order: each script takes as input the output of the previous one.



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align.pl
Align the reads from each sample on reference genome(s), convert output from SAM to BAM and sort the BAMs on-the-fly, then index them.


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callVariants.pl
Call variants with GATK HaplotypeCaller, producing a GVCF for each sample.


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mergeGVCFs.pl
Produce a single multi-sample GVCF per reference genome, usage example:
mergeGVCFs.pl ../CallVariants/*VEP.g.vcf > merged_ensembl.g.vcf 2> merged_ensembl.log


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gvcf2vcf.pl
Parse a merged GVCF, produce a VCF where non-informative lines and ALTs are removed and genotypes are called, example usage:
gvcf2vcf.pl < ../MergeGVCFs/merged_ensembl.g.vcf  > mergedVCF_ensembl.vcf 2> mergedVCF_ensembl.log


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filterVCF.pl
Find good candidates in the previous VCF, taking into account our knowledge of the various strains sequenced.
Examples:
# Good candidates for each strain (strict, ie don't allow NOCALLs)
filterVCF.pl --strain 1 --zero 0,1,3,5,6,7 --one 2,4  < ../GVCF2VCF/mergedVCF_ensembl.vcf > goodCandidates_ensembl_strain1_strict.vcf
filterVCF.pl --strain 2 --zero 0,1,2,4,5,7 --one 3,6  < ../GVCF2VCF/mergedVCF_ensembl.vcf > goodCandidates_ensembl_strain2_strict.vcf
# Good candidates for strain 1 (allow NOCALLs, need at least one true 0/0 and 1/1)
./filterVCF.pl --strain 1 --nocalls --zero 0,1,3,5,6,7 --one 2,4  < ../GVCF2VCF/mergedVCF_ensembl.vcf > goodCandidates_ensembl_strain1_allowNocalls.vcf


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