Calculates the melting temperature of a user imported forward and reverse primer based on the SantaLucia, 1998 salt correction formula used also in primer3 and primer blast. It can correct the tm calculation based on the existence of additives formamide & DMSO.
GUI executables are avaliable in:
Olga Tsiouri. (2023). Tm calculator GUI: Windows and ubuntu linux GUI apps that calculate the melting temperature of a user imported forward and reverse primer based on primer blast default parameters. Zenodo. https://doi.org/10.5281/zenodo.7978091
- python3.10
- anaconda/miniconda
- Gooey:
pip install Gooey - biopython:
pip install biopython