/msfragger2saint

Merges PPI outputs from MSFragger, generates the three files required by SAINT, and gives a general visualization of the data.

Primary LanguageRMIT LicenseMIT

msfragger2saint

This tool launches a local Shiny-based web application that generates the three files required by SAINT (http://proteomics.fi/), as a .zip file. The input can be either a single file (reprint.spc) or several ones that can be combined into one. The app also provides a general visualization of the data.

Requirements for the input file

A .tsv file.

The the output of FragPipe, usually named as "reprint.spc", which means that it has to include the following annotation columns: "PROTID", "GENEID", and "PROTLEN"; and the columns with the actual data (remember to keep the names as simple as possible). On the second line of the file, there should be the metadata / experiment information, as it was written on the Workflow tab of FragPipe.

For the app to work, it is mandatory that this information is present. In addition, keep in mind that, if the file/s have many entries, it will take a bit of time to generate the .zip file