Exact sample inference from Illumina amplicon data. Resolves real variants differing by as little as one nucleotide.
DADA2: High resolution sample inference from Illumina amplicon data. Nature Methods, 2016. (Open Access link.)
The dada2 package binaries are available through Bioconductor:
## try http:// if https:// URLs are not supported
source("https://bioconductor.org/biocLite.R")
biocLite("dada2")In order to install dada2 from source (and get the latest and greatest new features) see our installation from source instructions.
Our walkthrough of the DADA2 pipeline on paired end Illumina Miseq data.
The dada2 package manual.
Further documentation is available on the DADA2 front page.
Planned feature improvements are publicly catalogued at the main DADA2 development site on github; specifically on the "Issues" page for DADA2:
https://github.com/benjjneb/dada2/issues
If the feature you are hoping for is not listed, you are welcome to add it as a feature request "issue" on this page. This request will be publicly available and listed on the page.
Bugs and difficulties in using DADA2 are also welcome on the issue tracker.