/boggy

Real-Time PCR Data Sets by Boggy et al. (2010)

Primary LanguageRCreative Commons Attribution 4.0 InternationalCC-BY-4.0

boggy

CRAN status R-CMD-check

{boggy} provides the real-time PCR data set s1 by Boggy et al. (2010) in tidy format.

Installation

install.packages("boggy")

Usage

library(boggy)
library(ggplot2)

s1 |>
  ggplot(mapping = aes(
    x = cycle,
    y = fluor,
    group = well,
    col = format(copies, big.mark = ",", scientific = FALSE)
  )) +
  geom_line(linewidth = 0.2) +
  geom_point(size = 0.2) +
  labs(color = "Copy number")

References

Gregory J. Boggy & Peter J. Woolf. A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data. PLoS ONE 5:8 (2010). doi: 10.1371/journal.pone.0012355.