There has been a rapid uptake in the use of non-linear dimensionality reduction (NLDR) methods such as t-distributed stochastic neighbour embedding (t-SNE) in the natural sciences as part of cluster orientation and dimension reduction workflows. The appropriate use of these methods is made difficult by their complex parameterisations and the multitude of decisions required to balance the preservation of local and global structure in the resulting visualisation. We present visual diagnostics for the pragmatic usage of NLDR methods by combining them with a technique called the tour. A tour is a sequence of interpolated linear projections of multivariate data onto a lower dimensional space. The sequence is displayed as a dynamic visualisation, allowing a user to see the shadows the high-dimensional data casts in a lower dimensional view. By linking the tour to a view obtained from an NLDR method, we can preserve global structure and through user interactions like linked brushing observe where the NLDR view may be misleading. We display several case studies from both simulated and real data from single cell transcriptomics, that shows our approach is useful for cluster orientation tasks. The implementation of our framework is available as an R package called liminal available at https://github.com/sa-lee/liminal.
Package dependencies for the case studies can be installed with the BiocManager package
# install.packages("BiocManager")
BiocManager::install("sa-lee/paper-liminal")The case studies that produce the videos and still images found in the
paper can be reproduced in R via running the files found in the
scripts directory.
R> .
R> ├── DESCRIPTION
R> ├── README.Rmd
R> ├── README.md
R> ├── data
R> │ └── qd.rds
R> ├── img
R> ├── paper-liminal.Rproj
R> ├── paper.Rmd
R> ├── paper.tex
R> ├── scripts
R> │ ├── figures.R
R> │ ├── helpers.R
R> │ ├── liminal-gaussian.R
R> │ ├── liminal-mouse.R
R> │ ├── liminal-quickdraw.R
R> │ └── liminal-trees.R
R> ├── template
R> │ ├── JDSSV_editorialprocess.tex
R> │ └── jdssv_template.tex
R> └── video
R> ├── liminal-gaussian.mov
R> ├── liminal-hierachical.mov
R> ├── liminal-mouse-01.mov
R> ├── liminal-mouse-02.mov
R> ├── liminal-trees-01.mov
R> └── liminal-trees-02.mov