securecrt

advanced-go-programming-book

:books: 《Go语言高级编程》开源图书，涵盖CGO、Go汇编语言、RPC实现、Protobuf插件实现、Web框架实现、分布式系统等高阶主题(完稿)

android_kernel_htc_qsd8k

linux 2.6.38 for htc qsd8x50 devices

android_kernel_htcleo-2.6.32-ics

The first Android ICS kernel with most ICS features support for HTC HD2/Leo.

desire_kernel_35

A port of the HTC Desire 2.6.35 kernel to the HTC HD2

GA-H270M-D3H

OpenCore EFI for Gigabyte GA-H270M-D3H

GA-Z790-UD

hackintosh 13900 macos

linux-leo

2.6.32 kernel for leo with quite a lot of backported code

platform_vendor_qcom_proprietary

Proprietary stuff from CodeAurora

SecureCRT-HD2-2.6.32.X-HWA

this is the first HWA kernel for HD2

tiamat-leo

2.6.38 WIP

securecrt/GA-Z790-UD

hackintosh 13900 macos

securecrt/GA-H270M-D3H

OpenCore EFI for Gigabyte GA-H270M-D3H

securecrt/advanced-go-programming-book

:books: 《Go语言高级编程》开源图书，涵盖CGO、Go汇编语言、RPC实现、Protobuf插件实现、Web框架实现、分布式系统等高阶主题(完稿)

securecrt/Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273

RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.

securecrt/awesome-go

A curated list of awesome Go frameworks, libraries and software

securecrt/awesome-python

A curated list of awesome Python frameworks, libraries, software and resources

securecrt/BASE-EFI-INTEL-DESKTOP-13THGEN-RAPTOR-LAKE

Basic EFI with Opencore for Intel Raptor Lake Processors (13th Gen)

securecrt/cs-self-learning

计算机自学指南

securecrt/DTMA-YouTube

Chrome extension to remove the Youtube popUp to disable your adblock.

securecrt/everyone-can-use-english

人人都能用英语

securecrt/General-EFI-for-H610-B660-Z690-B760-Z790

General-EFI-for-600/700-Series Continually updated / 持续更新

securecrt/go-fundamental-programming

《Go 编程基础》是一套针对 Google 出品的 Go 语言的视频语音教程，主要面向新手级别的学习者。

securecrt/Introduction-to-Golang

【未来服务器端编程语言】最全空降golang资料补给包（满血战斗），包含文章，书籍，作者论文，理论分析，开源框架，云原生，大佬视频，大厂实战分享ppt

securecrt/IPTV

本项目共享江苏电信IPTV组播地址

securecrt/IPTV-1

Live TV m3u

securecrt/Lhy_Machine_Learning

李宏毅2021春季机器学习课程课件及作业

securecrt/luci-access-control

OpenWrt internet access scheduler

securecrt/luci-app-xray

(Almost) full feature Xray client for OpenWrt

securecrt/mydocker

Backup and share the dockerfile and docker-compose.yml

securecrt/ODH

A chrome extension to show online dictionary content.

securecrt/Paddle

PArallel Distributed Deep LEarning: Machine Learning Framework from Industrial Practice （『飞桨』核心框架，深度学习&机器学习高性能单机、分布式训练和跨平台部署）

securecrt/PaddleNLP

NLP Core Library and Model Zoo based on PaddlePaddle 2.0

securecrt/plunger

A Youtube adblock-block-un-blocker browser extension

securecrt/pro-go

Source Code for 'Pro Go' by Adam Freeman

securecrt/refined-github

:octocat: Browser extension that simplifies the GitHub interface and adds useful features

securecrt/sing-box

The universal proxy platform

securecrt/spider_collection

python爬虫，目前库存：网易云音乐歌曲爬取，B站视频爬取，知乎问答爬取，壁纸爬取，xvideos视频爬取，有声书爬取，微博爬虫，安居客信息爬取+数据可视化，哔哩哔哩视频封面提取器，ip代理池封装，知乎百万级用户爬虫+数据分析

securecrt/street-fighter-ai

This is an AI agent for Street Fighter II Champion Edition.

securecrt/superputty

The SuperPuTTY Window Manager for putty sessions

securecrt/tv