npp is a java script intended for the screening of KEPs. It will cleave all input protein sequences at the residues of KK,KR,RK and RR. Cleaved products are compared using blastp from blast+ command line toolkit to look for similar cleavage products (blast identity of 70%, resp 40% for very short sequences) For more detailed Methods, please see publication (coming soon).
Fasta file with protein sequences (Copci_AmutBmut1_GeneModels_FrozenGeneCatalog_20160912_aa.fasta) NOTE: Header sequence to split fasta file into protein sequences has been hardcoded and would need to be adapted for other input files.
Apache Commons Text library commons-text-1.0.jar Apache blastp from blast-2.7.1+ blast+
Script was originally run with blast-2.7.1+ and commons-text-1.0.jar.