Questions about sample.vcf in step1
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Hello,
I noticed that while using MuTect2 single model you recommend "PASS" vcfs as input for DeepMosaic.
Is the command parameter of MuTect2 single model similiar as "gatk Mutect2 -R reference.fa -I sample.bam -O single_sample.vcf.gz"? AND how to generate "PASS" vcfs?
Could you help me?
Hello, I noticed that while using MuTect2 single model you recommend "PASS" vcfs as input for DeepMosaic. Is the command parameter of MuTect2 single model similiar as "gatk Mutect2 -R reference.fa -I sample.bam -O single_sample.vcf.gz"? AND how to generate "PASS" vcfs? Could you help me?
Hi WZo0o
Thank you for your question, you can follow the following snakemake pipeline as an example.
The pipeline follows the GATK MuTect2 single mode official guides, starting from split intervals (if you have an entire genome to screen), run MuTect2, generated panel of normal, and run MuTect2 single mode, all the way down to extract filtered and passed.
Details are documented in https://gatk.broadinstitute.org/hc/en-us/articles/360037227652-CreateSomaticPanelOfNormals-BETA- and https://gatk.broadinstitute.org/hc/en-us/articles/360037593851-Mutect2
I will also update this in the main tutorial. Thank you again for your question! Please do not hesitate to reach out if you have any further questions!
All the best,
Xiaoxu
Hi, shishenyxx
Thanks for your help.
DeepMosaic is designed to run without control samples, but the GATK MuTect2 single-mode official guides seem still need unmatched control samples.
Is my point correct?
Hi, shishenyxx
Thanks for your help.
DeepMosaic is designed to run without control samples, but the GATK MuTect2 single-mode official guides seem still need unmatched control samples.
Is my point correct?
Hi,
Yeah if you are using GATK MuTect2 single mode it needs it, while if you are using GATK haolotypecaller ploidy 50 it doesn't; require a panel of normal.
Either could serve as input for DeepMosaic.
Best,
Xiaoxu
Hi, Xiaoxu
Which will be a better pipeline to serve as input for DeepMosaic between MuTect2 and haolotypecaller?
Best,
Zheng Wang
Hi, Xiaoxu
Which will be a better pipeline to serve as input for DeepMosaic between MuTect2 and haolotypecaller?
Best,
Zheng Wang
Hi Zheng,
From our own experience and others' preliminary data, MuTect2 single (tumor-only) mode has better performance.
Best,
Xiaoxu
Hi, Xiaoxu
Thanks for your reply.
Best,
Zheng Wang