desa_d_hydrogel-widefield-ORI

description of code and data for "Optical redox imaging to screen synthetic hydrogels for stem cell-derived cardiomyocyte differentiation and maturation" by DE Desa, MJ Amitrano, WL Murphy, MC Skala (https://doi.org/10.1117/1.BIOS.1.1.015002).

  • To reproduce plots, the included Excel files can be used ('data' folder).
  • Raw images can be provided upon request:
    • To reproduce 2-photon FLIM data, the cell-analysis-tools repository (https://github.com/skalalab/cell-analysis-tools) can be used. Dependencies and instructions can be found in the linked repository. Raw fluorescence decays and/or exponential fits can be provided as needed.
    • To generate widefield ORR, the ImageJ script can be run on each individual folder, which includes a matched NAD(P)H and FAD intensity tiff, available upon request.