Diabetes

Data Information

Samples Diabetes : 3 samples
Control : 3 Samples

Platform
Nanostring Ncounter Platform

Probes
Endogeneous : 798
HouseKeeping : 6
Ligation : 6

Data Analysis

Expression data was normalized using Nsolver Software

Differentially Expressed miRNA [DEmiRNA]

DEmiRNA : 51
Fold Change : 1.5
P-value : <= 0.05

DEmiRNA : 15
Fold Change : 2
P-value : <= 0.05

miRNA Target Interaction (MTI)

Experimentally Validated MTI was retrived from MiRTarBase
Experiments

Luciferase reporter assay
ELISA
Reporter assay
Flow
Western blot
qRT-PCR
Luciferase assay
Immunofluorescence
GFP reporter assay
Immunohistochemistry
Immunocytochemistry
In situ hybridization
Northern blot
Immunoprecipitaion
Immunoblot
Microarray
FACS
Chromatin immunoprecipitation
ChIP-seq
Proteomics
Flow cytometry
ChIP
EMSA

Filtering miRNA-targets

Support Type

Functional MTI
Functional MTI (Weak)
Non-Functional MTI
Non-Functional MTI (Weak)

Considered only Functional MTI from the Support Type

Functional Enrichment Analysis

The targets of DEmiRNAs for given for functional enrichment.

The ontologies were further filtered with keywords:

insulin
glucagon
glucose
lipid