Fold gene expression analysis, for Bio-Rad CFX qPCR output.
Takes a path to an Excel spreadsheet, a reference sample/cell line and a reference target (i.e. housekeeping gene) as input.
- numpy
- pandas
- matplotlib
# Will create a file titled
# "Analysis - HEK293T - Quantification Summary.csv"
# Treats "HEK293T parental" as the reference sample,
# and "18S" as the reference target, for all samples
./foldch.py \
--rs all "HEK293T parental" \
--rt all "18S" -- \
"HEK293T - Quantification Summary.csv"
# Will use "Untreated 4h" as the reference sample
# for all samples ending in 4h, and similarly for 12h
./foldch.py \
--rt all GAPDH \
--rs /.+ 4h/ "Untreated 4h" \
--rs /.+ 12h/ "Untreated 12h" \
-o analysis.csv -- \
*Quantification\ Summary*.csv
./foldch.py ...
./barchart.py "Analysis - HEK293T - Quantification Summary.csv"