Demonstration of fitting Time-Correlated Single Photon Counting (TCSPC) data with consideration of the Instrument Response Function (IRF).
Note that the example data included in this repository is artificial, although its appearance is based on that of a real measurement. The IRF is real, and has been recorded on a Edinburgh Instruments FS5 Spectrofluorometer.
For further reading on TCSPC and IRF fitting see the following:
-
Tesa et al., "Measurement of Photoluminescence lifetimes in the μs Range", Technical Note from Edinburgh Instruments, TN_P48, 31 May 2018
-
Schönau et al., "Performing high speed lifetime measurements of proteins using a 280 nm picosecond laser", Application Note from PicoQuant, 2019
-
TauFit - TCSPC module of PAM software
-
"What is Time-Correlated Single Photon Counting?", Blog post from Edinburgh Instruments
-
Wahl et al., "Time-Correlated Single Photon Counting", Technical Note from PicoQuant (2014)
-
Léonard et al. "High-throughput Time-Correlated Single Photon Counting", Lab on a Chip, 14, 4338-4343, (2014) as well as the Supplementary Information
-
O'Connor et al. "Deconvolution of fluorescence decay curves. A critical comparison of techniques", J. Phys. Chem., 83, 10, 1333–1343 (1979)
-
Kalisz, Józef "Review of methods for time interval measurements with picosecond resolution", Metrologia 41 17 (2004)