This MATLAB code provides an easy way to simulate TCSPC image data which includes effect of the IRF, background after-pulsing and laser repetition rate. Images are saved as OME.tiff using Bioformat.
The method uses a Monte-Carlo simulation of photon arrival times based on probability density functions of emission (exponential) and excitation (Gaussian).
The OME.tiff save was kindly provided by Ian Munro i.munro@imperial.ac.uk from the Photonics Group at Imperial College, London. Thanks to the OME team OME team who maintains the Bio-Formats library. Contact: Sebastien Besson s.besson@dundee.ac.uk from Dundee.
You need to download the Bio-formats Matlab plugin from http://downloads.openmicroscopy.org/bio-formats/ and set the path to it as shown in the file.