Annotation Tutorial

Example code and data to accompany : [link to paper]

Requirements

R 4.0.2 or higher. Development version of Bioconductor

R packages: ggplot2, harmony, Seurat, SingleR, scater, SingleCellExperiment, scmap, celldex, fgsea, gsva?, SCINA, scCATCH

RStudio is recommended.

Set Up

Before running the tutorial, it is necessary to uncompress the raw data (pbmc3k_filtered_gene_bc_matrices.tar.gz) with the resulting folders in the same directory as the tutorial Rmd file.

The Rmd file has been tested in RStudio on various platforms.