scRNA-seq QC
performs quality-control on single-cell RNA-seq count data and returns the counts of the filtered cells and genes.
Input projection | . |
---|---|
y-axis |
numeric, count data |
columns |
character, cell ID or name |
rows |
character, gene name |
Output relations | . |
---|---|
percent_mt |
numeric, percent counts mapping to mitochondrial genes per cell |
nCount_RNA |
numeric, total counts per cell |
nFeature_RNA |
numeric, number of features (genes) detected per cell |
The operator uses the Seurat
R package and the QC workflow described in the "package website".
Hao, Y., Hao, S., Andersen-Nissen, E., Mauck, W. M., Zheng, S., Butler, A., ... & Satija, R. (2021). Integrated analysis of multimodal single-cell data. Cell, 184(13), 3573-3587.