primer-design

There are 54 repositories under primer-design topic.

  • poly

    bebop/poly

    A Go package for engineering organisms.

    Language:Go7131216571

  • tao-bioinfo/PrimerServer2

    PrimerServer2: a high-throughput primer design and specificity-checking platform

    Language:Python7851514

  • widdowquinn/find_differential_primers

    Code for design of diagnostic PCR primers, and metabarcoding markers.

    Language:Python6283325

  • gear-genomics/dicey

    In-silico PCR, primer design and padlock design for in-situ sequencing

    Language:C++515144

  • biologger/speciesprimer

    The SpeciesPrimer pipeline is intended to help researchers finding specific primer pairs for the detection and quantification of bacterial species in complex ecosystems.

    Language:Python4533318

  • jonas-fuchs/varVAMP

    Design degenerated primers on highly variable alignments for full genome sequencing or qPCR. Specifically developed for viruses.

    Language:Python416712

  • VascoElbrecht/PrimerMiner

    R mased batch sequence downloader, with primer development and in silico evaluation capabilities

    Language:R3664111

  • Edinburgh-Genome-Foundry/Primavera

    :cherry_blossom: Python library for primer-based verification of DNA assemblies: primer selection, data analyis, etc.

    Language:Python33904

  • dieterich-lab/circtools

    circtools: a modular, python-based framework for circRNA-related tools that unifies several functionalities in a single, command line driven software.

    Language:Python3157321

  • github/mlops

    Use GitHub to facilitate automation, collaboration and reproducibility in your machine learning workflows.

    Language:JavaScript26227

  • ujenjt/miprimer

    Webapp for design DNA primers for miRNA qPCR process

    Language:CSS15102

  • fulcrumgenomics/prymer

    Python Primer Design Library

    Language:Python125530

  • smitbarmase/pods

    project management and communication tool built with react, node and graphql

    Language:TypeScript101142

  • tao-bioinfo/PrimerServer

    Obsolete!! Begin to turn to PrimerServer2

    Language:Perl101382

  • gear-genomics/silica

    In-silico PCR amplification on complete genomes

    Language:JavaScript9410
  • primerdriver

    kvdomingo/primerdriver

    PrimerDriver - Automated design of mutagenic PCR primers

    Language:Python8100

  • Owaiskhan9654/Gene-Sequence-Primer-

    Gene and Primer Sequence Analysis for SARS-CoV-2, EGFR(Non Small Lung Cancer Cell), Influenza DNAs ### How can I check my Oligo primers to ensure there are no significant primer design issues? - The difference between melting temperatures (Tm) of the primers should be less than 5°C. - The GC content should be between 35-80% or equivalent to the product being amplified. - The Delta G value of any self-dimers, hairpins, and heterodimers should be weaker (more positive) than -9.0 kcal/mole. Positive numbers indicate that the actual secondary structure shown will not form at all. - Avoid 3' complementarity between the two primers to prevent primer dimers. The IDT OligoAnalyzer APIs can be used to assess these different criteria for a proposed oligo. #### [Reference](https://sg.idtdna.com/pages/support/faqs/how-can-i-check-my-pcr-primers-using-the-oligoanalyzer-program-to-ensure-there-are-no-significant-primer-design-issues-)

    Language:Jupyter Notebook8101

  • rdpstaff/EcoFunPrimer

    primer design pipeline

    Language:Java8854

  • ipb-halle/GoldenMutagenesis

    Support for rapid design of primers for amino acid exchanges and saturation mutagenesis by Golden Gate cloning.

    Language:R54173

  • qPCR4vir/ThDySec

    Modeling of primers/probes hybridization on to a set of target viral sequences.

    Language:C++41431

  • weinerlab/mtk_primer_generator

    Automagically ✨ generate primers for Golden Gate cloning using the Mammalian Cloning Toolkit (MTK)

    Language:Python4266

  • DanielSchuette/cloningPrimer

    Web App, CLI, and API for designing cloning primer pairs - written in Go👩🏼‍🔬👨🏼‍🔬

    Language:Go3102

  • gear-genomics/verdin

    Variant Primer Design

    Language:Python3400

  • Mohamed-Elmanzalawi/COVID-19-PCR-primer-design

    Streamlining dataset analysis and primer design for efficient genetic studies. Our Project is driven by robust algorithms, aims to achieve quick and accurate results. Leveraging Muscle alignment and Primer-BLAST, the project simplifies the process to enhance the precision of genetic analysis.

    Language:Python3100

  • caanene1/miRNAprimer

    R package for designing miRNA primers.

    Language:R2102

  • Kallepan/primer-designer

    Primer Design Tool

    Language:Python2141

  • sanjaynagi/AnoPrimer

    Primer Design in Anopheles gambiae and funestus considering genetic variation

    Language:Jupyter Notebook22334

  • ThWacker/DiPPER2

    DiPPER2: Diagnostic Primer Picking and Evaluation pipeline for Reliability and Reproducibility

    Language:Python2100

  • AntoineHo/PriDe

    PriDe: Primer Design tool

    Language:Python1102

  • hectoraliagam/primer-repositorio

    Primer repositorio creado en github con mi cuenta, modo learn-try

    Language:HTML1110

  • lerethel/primer-search

    A simple primer design tool that fetches data from Ensembl and Primer-BLAST. Runs on a Node.js server and supports Docker

    Language:JavaScript1100

  • leylabmpi/CoreGenomePrimers

    Design clade-specific primers targeting a pan-genome core gene

    Language:Jupyter Notebook1341

  • padrong/TakaraGxl_Calculator

    A calculator written in python 3 based off the takara GXL polymerase protocol. Used to calculate Tm.

    Language:Python1100

  • ShuXingYu94/Primer-picker

    Designing and blasting to find primer with specific PCR product

    Language:Python1200

  • swiing/hugo-theme-primer

    Makes it easy to use github primer in hugo

    Language:HTML1100

  • tripitakit/aliscan

    Aliscan helps to identify signature patterns in nucleotide sequences alignments

    Language:HTML00