primer-design
There are 51 repositories under primer-design topic.
bebop/poly
A Go package for engineering organisms.
billzt/PrimerServer2
PrimerServer2: a high-throughput primer design and specificity-checking platform
widdowquinn/find_differential_primers
Code for design of diagnostic PCR primers, and metabarcoding markers.
gear-genomics/dicey
In-silico PCR, primer design and padlock design for in-situ sequencing
biologger/speciesprimer
The SpeciesPrimer pipeline is intended to help researchers finding specific primer pairs for the detection and quantification of bacterial species in complex ecosystems.
Edinburgh-Genome-Foundry/Primavera
:cherry_blossom: Python library for primer-based verification of DNA assemblies: primer selection, data analyis, etc.
VascoElbrecht/PrimerMiner
R mased batch sequence downloader, with primer development and in silico evaluation capabilities
jonas-fuchs/varVAMP
Design degenerated primers on highly variable alignments for full genome sequencing or qPCR. Specifically developed for viruses.
dieterich-lab/circtools
circtools: a modular, python-based framework for circRNA-related tools that unifies several functionalities in a single, command line driven software.
github/mlops
Use GitHub to facilitate automation, collaboration and reproducibility in your machine learning workflows.
ujenjt/miprimer
Webapp for design DNA primers for miRNA qPCR process
billzt/PrimerServer
Obsolete!! Begin to turn to PrimerServer2
0xtimsb/pods
🚀 Project management and communication tool for teams.
gear-genomics/silica
In-silico PCR amplification on complete genomes
kvdomingo/primerdriver
PrimerDriver - Automated design of mutagenic PCR primers
fulcrumgenomics/prymer
Python Primer Design Library
Owaiskhan9654/Gene-Sequence-Primer-
Gene and Primer Sequence Analysis for SARS-CoV-2, EGFR(Non Small Lung Cancer Cell), Influenza DNAs ### How can I check my Oligo primers to ensure there are no significant primer design issues? - The difference between melting temperatures (Tm) of the primers should be less than 5°C. - The GC content should be between 35-80% or equivalent to the product being amplified. - The Delta G value of any self-dimers, hairpins, and heterodimers should be weaker (more positive) than -9.0 kcal/mole. Positive numbers indicate that the actual secondary structure shown will not form at all. - Avoid 3' complementarity between the two primers to prevent primer dimers. The IDT OligoAnalyzer APIs can be used to assess these different criteria for a proposed oligo. #### [Reference](https://sg.idtdna.com/pages/support/faqs/how-can-i-check-my-pcr-primers-using-the-oligoanalyzer-program-to-ensure-there-are-no-significant-primer-design-issues-)
rdpstaff/EcoFunPrimer
primer design pipeline
ipb-halle/GoldenMutagenesis
Support for rapid design of primers for amino acid exchanges and saturation mutagenesis by Golden Gate cloning.
qPCR4vir/ThDySec
Modeling of primers/probes hybridization on to a set of target viral sequences.
weinerlab/mtk_primer_generator
Automagically ✨ generate primers for Golden Gate cloning using the Mammalian Cloning Toolkit (MTK)
DanielSchuette/cloningPrimer
Web App, CLI, and API for designing cloning primer pairs - written in Go👩🏼🔬👨🏼🔬
gear-genomics/verdin
Variant Primer Design
Mohamed-Elmanzalawi/PCR-primer-script
Streamlining dataset analysis and primer design for efficient genetic studies. Our Project is driven by robust algorithms, aims to achieve quick and accurate results. Leveraging Muscle alignment and Primer-BLAST, the project simplifies the process to enhance the precision of genetic analysis.
sanjaynagi/AnoPrimer
Primer Design in Anopheles gambiae and funestus considering genetic variation
AntoineHo/PriDe
PriDe: Primer Design tool
caanene1/miRNAprimer
R package for designing miRNA primers.
eskutkaan-zz/biodata
a molecular solutions toolbox for molecular biologists.
GEizaguirre/PolyPrimer
Tool in Perl for finding Polymorphisms in primers for human genes.
hectoraliagam/primer-repositorio
Primer repositorio creado en github con mi cuenta, modo learn-try
Kallepan/primer-designer
Primer Design Tool
leylabmpi/CoreGenomePrimers
Design clade-specific primers targeting a pan-genome core gene
swiing/hugo-theme-primer
Makes it easy to use github primer in hugo
rahriver/PrimerX
Simple shiny app to analyze primer quality.
simonmichau/primertool
Streamlit app for generating primers for PCR/Sanger sequencing based on genomic variants. Visit https://primertool.simonmichau.de/ for the deployed tool. Documentation available under https://simonmichau.github.io/primertool/