trumb

aws-cudahashcat-auto

Automated password cracking with AWS GPU instances and CUDA hashcat

BlackICE_Connect

CudaTree

Building random forests on GPU

EquationGroupLeak

Archive of leaked Equation Group materials

goatrider

GoatRider is a simple tool that will dynamically pull down Artillery Threat Intelligence Feeds, TOR, AlienVaults OTX, and the Alexa top 1 million websites and do a comparison to a hostname file or IP file.

lantern-compute-pcb

Files related to Lantern's main PCB, such as schematic, Gerbers, and BOM

OneNoteAPISampleWinStore

Code sample with examples of how to use the OneNote API for Windows 8 apps

PowerShell

Useful PowerShell scripts

PowerShellArsenal

A PowerShell Module Dedicated to Reverse Engineering

ridenum

Rid_enum is a null session RID cycle attack for brute forcing domain controllers.

trumb/BlackICE_Connect

trumb/EquationGroupLeak

Archive of leaked Equation Group materials

trumb/goatrider

GoatRider is a simple tool that will dynamically pull down Artillery Threat Intelligence Feeds, TOR, AlienVaults OTX, and the Alexa top 1 million websites and do a comparison to a hostname file or IP file.

trumb/lantern-compute-pcb

Files related to Lantern's main PCB, such as schematic, Gerbers, and BOM

trumb/PowerShell

Useful PowerShell scripts

trumb/ridenum

Rid_enum is a null session RID cycle attack for brute forcing domain controllers.

trumb/artillery

The Artillery Project is an open-source blue team tool designed to protect Linux and Windows operating systems through multiple methods.

trumb/Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273

RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.

trumb/auto-ossec

trumb/azure-quickstart-templates

Azure Quickstart Templates

trumb/CNTK

Computational Network Toolkit (CNTK)

trumb/CryptoTools

Tools for attacking cryptographic implementations.

trumb/csharp_with_csharpfritz

Show notes, slides, and samples from the CSharp with CSharpFritz show

trumb/docker

Docker - the open-source application container engine

trumb/dsc-generator

PowerShell Desired State Configuration (DSC) Generator

trumb/exploit-database

The official Exploit Database repository

trumb/generative_agents

Generative Agents: Interactive Simulacra of Human Behavior

trumb/iomemory-vsl

Updated Fusion-io iomemory VSL Linux (version 3.2.16) driver for recent kernels.

trumb/KML

Kerbal Markup Lister - A persistence file editor for Kerbal Space Program

trumb/losslessh264

Open Source H.264 Codec

trumb/pla1750b

trumb/polarbearrepo

trumb/Red_Team

Some scripts useful for red team activities

trumb/social-engineer-toolkit

The Social-Engineer Toolkit (SET) repository from TrustedSec - All new versions of SET will be deployed here.

trumb/spreadsheets-are-all-you-need

trumb/Terminus2049.github.io

No more 404

trumb/tintri-powershell-examples

Examples of using the Tintri Automation Toolkit for PowerShell.

trumb/WaveFunctionCollapse

Bitmap & tilemap generation from a single example with the help of ideas from quantum mechanics

trumb/youtube-dl

Command-line program to download videos from YouTube.com and other video sites

trumb/youtube-dl-gui

A cross platform front-end GUI of the popular youtube-dl written in wxPython.