Bowtie Problem

[liniguez]

I have added the bin folder to my path and I get an error message about bowtie:

`
/users/mirimia/projects/vast-tools/vast-tools align test_1.fastq test_2.fastq --name testOpossum --cores 1 --noIR --sp monDom5
[vast align]: VAST-TOOLS v2.5.1
[vast align]: Species assembly: monDom5, VASTDB Species key: Mdo
[vast align]: VASTDB Version: vastdb.mdo.23.06.20
Argument "400000 /nfs/users/mirimia/liniguez/test_vasttools/test_1..." isn't numeric in division (/) at /nfs/users/mirimia/projects/vast-tools/bin/RunDBS_1.pl line 432.
Argument "400000 /nfs/users/mirimia/liniguez/test_vasttools/test_2..." isn't numeric in division (/) at /nfs/users/mirimia/projects/vast-tools/bin/RunDBS_1.pl line 433.
[vast align]: Input RNA-seq file(s): /nfs/users/mirimia/liniguez/test_vasttools/test_1.fastq and /nfs/users/mirimia/liniguez/test_vasttools/test_2.fastq
[vast align]: Most common read lengths detected for fq1 & fq2: 101 (100.00%) and 101 (100.00%)
[vast align]: Sample name: testOpossum
[vast align]: Using VASTDB -> /nfs/users/mirimia/projects/vast-tools/VASTDB/Mdo
[vast align]: Setting output directory to /nfs/users/mirimia/liniguez/test_vasttools/vast_out
[vast align]: Setting tmp directory..
[vast align]: Set tmp directory to /nfs/users/mirimia/liniguez/test_vasttools/vast_out/tmp!
/users/mirimia/projects/vast-tools/bin/bowtie:24: DeprecationWarning: the imp module is deprecated in favour of importlib; see the module's documentation for alternative uses
import imp
Traceback (most recent call last):
File "/users/mirimia/projects/vast-tools/bin/bowtie", line 70, in
main()
File "/users/mirimia/projects/vast-tools/bin/bowtie", line 42, in main
bld = imp.load_source('bowtie-build', os.path.join(ex_path, 'bowtie-build'))
File "/users/mirimia/liniguez/miniconda3/lib/python3.8/imp.py", line 171, in load_source
module = _load(spec)
File "", line 702, in _load
File "", line 671, in _load_unlocked
File "", line 839, in exec_module
File "", line 975, in get_code
File "/users/mirimia/liniguez/miniconda3/lib/python3.8/imp.py", line 157, in get_data
return super().get_data(path)
File "", line 1032, in get_data
FileNotFoundError: [Errno 2] No such file or directory: '/nfs/users/mirimia/projects/vast-tools/bin/bowtie-build'
Too few first reads (<500) could be mapped to mRNA library for detecting strand-orientation of reads. Maybe wrong species selected? at /nfs/users/mirimia/projects/vast-tools/bin/RunDBS_1.pl line 599.

`