On white background image not colored

[NikoLichi]

Hi Wouter,

I am trying to find the best way to see matching colors and have tried both white and black backgrounds. However, with the same setup on a white background, there are no colors; please see the attached figure.

Is this a bug?

The code I am using is:

./kyber -t 16 \
--input $FILEIN_1 $FILEIN_2 \
--phred \
--background white \
--color red blue \
--output ${PREFIX}_GeneCvsNovo.png

Thanks and all the best,
Niko

[NikoLichi]

A small add-on: when using the --normalize option instead of --phred, the code works well.
Please see figure

Would you recommend the phred or the normalized option to check technical samples?

Best,
Niko

[wdecoster]

Hmm, this looks like a bug. Just to be sure, everything is alright when you use the black background?

[NikoLichi]

Yes, everything is normal see the attached figure.

Do you have any suggestions on the phred or the normalized option to check technical replicates?

[NikoLichi]

Hi Wouter,

I was checking the files again and discovered that what I thought was black is actually a high saturation. I meant, if you make Zoom on the image with white background it is possible to see the red, blue and purple colors.

However, I would think that even in high saturation the overlapping colors should be kept, in this case purple and not black, or?

So, I guess Kyber is doing what it should! But the issue with the black color of overlapping points is weird to me.

All the best,
Niko

[wdecoster]

Finally fixed, will make a new release soon

[NikoLichi]

This is great! Thanks, Wouter.

[wdecoster]

Please let me know if you have other feedback :)