Multi-omic
zhangqc723 opened this issue · 1 comments
zhangqc723 commented
Hello,
Now, I had got the output form 10X Multi-omic (RNA+ATAC) by cellranger-arc, and I want to use velocyto run10x for my RNA data form 10X RNA+ATAC. What should I do?
Thanks
danielee0707 commented
Please follow Velocyto's documentation to run their CLI tool. See http://velocyto.org/velocyto.py/tutorial/cli.html#run10x-run-on-10x-chromium-samples.
If that doesn't work on Multiome, you can also try directly calling Velocyto run http://velocyto.org/velocyto.py/tutorial/cli.html#run-run-on-any-technique-advanced-use using the 10X barcode file for --bcfile argument and gex_possorted_bam.bam as the input BAM file.