The number of SupportReads is not integer
Closed this issue · 2 comments
Bathroomboss commented
Dear Dr. Tianxiong,
TEMP2 is very useful and recently I used it to process my data and got the output file -- ${File}.insertion.bed, but I noticed something puzzled me. In the eighth column, which represents the support reads, there seems to appear decimals.
The pictures below are some examples.
I would appreciate it if you could explain the reason behind the appearance of a decimal point in this field.
Best wishes
Liang yu
tianxiongbb commented
Hi Liang,
Sometimes a read pair can support multiple transposon insertions due to
homology, e.g. gypsy and gypsy2; or aluY and aluYa. In this case, a read is
counted as 1 / number of supporting TEs for each supported transposon.
Best,
Tianxiong
…On Tue, Apr 11, 2023, 3:36 AM Bathroomboss ***@***.***> wrote:
Dear Dr. Tianxiong,
TEMP2 is very useful and recently I used it to process my data and got the
output file -- *${File}.insertion.bed*, but I noticed something puzzled
me. In the eighth column, which represents the support reads, there seems
to appear decimals.
*The pictures below are some examples.*
[image: image]
<https://user-images.githubusercontent.com/113091536/231083252-e7ee4f96-c8ec-4469-abdf-c5cb0c40bab0.png>
[image: image]
<https://user-images.githubusercontent.com/113091536/231083967-742520e4-7222-4274-a492-08c7d1d37006.png>
I would appreciate it if you could explain the reason behind the
appearance of a decimal point in this field.
Best wishes
Liang yu
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Bathroomboss commented
Thank you!