/reanalysis1

Original data and plots used for reanalysis purposed

Primary LanguagePerl

reanalysis1

Original data and codes uesd for reanalysis purposed


File descriptions


Fig1a,b, FigS1a~g

These folders contain source data and codes used for producing each figure and supplementary figures


igv_snapshots

Example of BAM alignments (against TAIR10 reference) shown in IGV (http://www.broadinstitute.org/software/igv/), the File A2.example_snapshots_of_homomeric_associated_mutations.v2.pptx gives examples of biased strands, more snapshots for problematic clustered "mutations" are given in clustered_somatic_mutations


equilibrium

Code for the dinucleotide equilibrium analysis


Notes:

(1) TEs are annotated based on "TAIR10_GFF3_genes_transposons.gff", so a few more mutations were annotated as TEs than labeled in original Weng's data. But no apparent influence was found in all analysis.

(2) Raw fastq files were downloaded from EBI (PRJNA434660:SRR6862404-SRR6862510). BAM files were generated using BWA-mem (version 0.7.10-r789), and were sorted and processed with MarkDuplicates from picard-tools (version 1.114), and further realigned with GenomeAnalysisTK (version 3.7).