remove PCR duplicate
yx-xu opened this issue · 2 comments
yx-xu commented
Hi!
I'm sorry to bother you. I followed Ribocoed workflow, but I don't know whether need to remove PCR duplicate after STAR alignment. Could you give me some suggestions? Thank you very much!
zhengtaoxiao commented
If you only want to identify the novel translating ORFs, PCR duplication removal is not necessary.
PCR duplications might influence the quantification of the translational level of ORFs。
yx-xu commented
Get it! Thanks for your reply!