EasyFuse is a pipline for fusion gene detection from RNA-seq data. More detailed documentation is available in the EasyFuse Wiki.
A manuscript describing the method and performance evaluations is submitted for peer-review and publication.
Fusion breakpoint prediction itself is currently not implemented in EasyFuse and the pipeline therefore depends on external fusion prediction tools.
Prediction tools that have been implemented and tested within EasyFuse are listed under Tools. EasyFuse requires STAR for alignments. Additional alignment tools might be required depending on the external fusion prediction tools.
For simplicity we provide in the following an installation instruction for EasyFuse together with STAR-Fusion and Fusioncatcher. Detailed installation instructions can be found in the EasyFuse Wiki
- star-fusion (1.5.0)
- fusioncatcher (1.00)
- mapsplice2 (2.2.1)
- infusion (0.8)
- SOAPfuse (1.2.7)
- pizzly (0.37.3)
- bowtie2 (2.3.4.3)
- kallisto (0.44.0)
- skewer (0.2.2)
- gzip (>=1.6) (if mapsplice shall be used)
- samtools (1.9)
- star (2.6.1d)
- Python (2.7.15)
- Python modules:
- pandas (0.24.0)
- matplotlib (2.2.3)
- seaborn (0.9.0)
- pysam (0.15.2)
- crossmap (0.2.7) (optional if liftover shall be included)
- biopython (1.73)
- xlrd (1.0.0)
- openpyxl (2.5.0a2)
- bx-python (0.8.2)
Install python modules (we strongly recommend installation via conda):
/path/to/conda/bin/conda create -n easyfuse python=2.7.15
source /path/to/conda/bin/activate easyfuse
conda install -c conda-forge pandas=0.24.0 matplotlib=2.2.3 seaborn=0.9.0 biopython=1.73 xlrd=1.0.0 openpyxl=2.5.0a2
conda install -c bioconda pysam=0.15.2 star=2.6.1b star-fusion=1.5.0 bowtie2=2.3.4.3 bx-python=0.8.2 crossmap=0.2.7
- R (>= 3.6.0)
- R packages:
- optparse (1.6.4)
- tidyverse (1.3.0)
- randomForest (4.6-14)
Install packages within R by
install.packages(c("optparse", "tidyverse", "randomForest"))
Before executing the pipeline some configuration files need to be adopted:
- rename
build_env.sh.smaple
intobuild_env.sh
and configure content. - rename
config.py.smaple
intoconfig.py
and configure content. - rename
blacklist.txt.sample
intoblacklist.txt
.
To start the fusion prediction pipeline on a specific sample the following python script has to be executed with the given input parameters as command-line arguments.
processing.py \
-i <sample_folder> \
-o <working_dir>
python processing.py -i test_case/SRR1659960_05pc_* -o test_easyfuse_1.3.4/
The output of EasyFuse is described in the wiki page EasyFuse Output.