Sequences Length
Closed this issue · 2 comments
ChristosMatzoros commented
Hello,
Is there a way to determine the length of the sequences in the generated file? For example to give a minimum or a maximum length?
Regards
yukiteruono commented
Thank you for your using PBSIM.
The length of reads is determined by a gamma distribution with a given mean and SD (--length-mean
and --length-sd
).
The length range can be roughly limited by --length-min
and --length-max
.
In template sequencing mode, the length of reads is approximately the same as the length of templates.
In multi-sequencing mode, the length of reads is approximately equal to --length-mean
.
PBSIM cannot generate a read with exactly the specified length. To do so, simulate reads and then cut them to the length you want.
ChristosMatzoros commented
Thank you