Lysosomes are critical for cellular metabolism and heterogeneously involved in various cellular
processes such as endocytosis, autophagy and senescence. The ability to measure lysosomal
metabolic heterogeneity is essential for understanding its physiological roles. We therefore
built a single-lysosome mass spectrometry (SLMS) platform integrating lysosomal patch-clamp
recording and induced nanoESI/MS that enabled concurrent metabolic and electrophysiological
profiling of individual enlarged lysosomes.
- xcms (version 3.9.1)
- Framework for processing and visualization of single-spectra mass spectral data.
- Seurat (version 3.2.0)
- Identify the main lysosome types and visualize the clustering information in 2D space by t-SNE.
- monocle (version 2.16.0)
- Analyze lysosome trajectories by sorting lysosomes in pseudo-time trajectories.
- FactoMineR (version 2.3), factoextra (version 1.0.7)
- Perform principal components analysis (PCA) to check if there is a batch effect in data.
- pheatmap (version 1.0.12)
- Plot heatmaps and perform hierarchical cluster analysis (HCA) to check if there is a batch effect in data.
- find peaks.r
- Preprocess the original data and get matrices with row names (m/z) and column names (sample names) after preprocessing.
- statistical analysis.R
- Normalize the intensity of each lysosome, check the batch effect and differential analysis.
- seurat.R
- Identify the main lysosome types and visualize the clustering information in 2D space by t-SNE.
- monocle.R
- Analyze lysosome trajectories by sorting lysosomes in pseudo-time trajectories.
- plot_peaks.R
- Map the average mass spectra for each subpopulation of lysosomes.
The mass spectrometric raw data of single lysosomes have been deposited to the MassIVE database under accession code MSV000087208 and can be visualized with Thermo Xcalibur Qual Browser.