RICS notes
RICS calculations on confocal image stacks of beads (2D + t).
Check the notebook on nbviewer, over at: https://nbviewer.jupyter.org/github/zindy/RICS/blob/master/RICS_calculation.ipynb
or have a play with the code over on Binder at: https://mybinder.org/v2/gh/zindy/RICS/master?filepath=RICS_calculation.ipynb
The following test images (100nm beads) are provided for testing (the original LIF files were converted to TIFF in FIJI / ImageJ):
- 050917 40nm 4.8us 100nm beads 20 scan v2.tif : https://github.com/zindy/RICS/raw/master/050917%2040nm%204.8us%20100nm%20beads%2020%20scan%20v2.tif
- 050917 40nm 4.8us 100nm beads 20 scan v3.tif : https://github.com/zindy/RICS/raw/master/050917%2040nm%204.8us%20100nm%20beads%2020%20scan%20v3.tif
[1] Brown, C. M. et al. “Raster Image Correlation Spectroscopy (RICS) for Measuring Fast Protein Dynamics and Concentrations with a Commercial Laser Scanning Confocal Microscope.” Journal of microscopy 229.0 1 (2008): 78–91. PMC. Web. 19 Sept. 2017.