Here we bring the scritps used to test parameters from 16S analysis from Ion Torrent data. We compared USEARCH and VSEARCH, SILVA, Greengenes and RDP reference databases, UCLUST and RDP classifiers, confidence levels and QIIME1 and QIIME2, chimera trimming steps and chimera trimming reference databases. See details and references in our manuscript that will be available soon.
Tiago Falcon - Centro de terapia Gênica, Hospital de Clínicas de Porto Alegre
I thank to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the fellowship provided to TF (process number: 156137/2018-6)