/taxcollector

16S rRNA database

Primary LanguagePythonMIT LicenseMIT

TaxCollector

Austin G. Davis-Richardson


For the original TaxCollector (publication), please check out the "publication" branch.

About

TaxCollector is a tool for modifying the Ribosomal Database Project (RDP) 16S rRNA database. The RDP database is a fasta file with 16S rRNA sequences. The headers are terribly non-descriptive. Using the NCBI names and nodes databases, this script can create detailed taxonomic descriptions.

For example, this script takes the default RDP header:

>S001018666 uncultured Acidobacteria bacterium; 2YMLF03; EF630306

And converts it to this:

>[0]Bacteria;[1]Acidobacteria;[2]Holophagae;[3]Holophagales;[4]Holophagaceae;[5]Geothrix;[6]Geothrix_fermentans;[7]Geothrix_fermentans[8]Geothrix_fermentans_(T)|U41563

Notice that the Genus is in "quotes." This means that there was no entry for Genus in the NCBI names and nodes databases. In this case, TaxCollector will make one up, recycling the previous name. This only happens for Genus and Order.

This can be useful for some people. For others, not.

By default, TaxCollector filters out entries with the following in their taxonomy:

'eukaryota'
'eukarya'
'clone'
'plasmid'
'vector'
'uncultured'

If you don't want this, change it in the taxcollector.py file

Invocation

Requirements

  • Python 3.7

For the lazy:

Type make.

This will download necessary databases and compile you a taxcollector.fa file

Otherwise

invoke comme ca

python taxcollector.py names.dmp nodes.dmp input.fasta > output.fasta

You can get names.dmp and nodes.dmp from NCBI's FTP site.

My favorite way to do this is by typing

curl ftp://ftp.ncbi.nih.gov/pub/taxonomy/taxdump.tar.gz | gunzip | tar -xvf names.dmp nodes.dmp

The FASTA file must come from RDP (http://rdp.cme.msu.edu).

License

The MIT License (MIT) Copyright (c) 2010-2018 Giongo A., Davis-Richardson A.G., Crabb D.B., Triplett E.W.

Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions:

The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software.

THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.

Please Cite: Giongo A., Davis-Richardson A.G., Crabb D.B., Triplett E.W. TaxCollector: Modifying Current 16S rRNA Databases for the Rapid Classification at Six Taxonomic Levels. Diversity. 2010; 2(7):1015-1025.